The High-Throughput Genomics (HTG) Shared Resource provides experimental support for diverse genomic applications that utilize the Illumina sequencing platform. The Resource operates as a full-service facility that offers expertise in all aspects of the experimental process including evaluation of sample quality, construction of Illumina sequencing libraries (genomic DNA, exome and targeted enrichment, methylation analysis, ChIP-seq, RNA-Seq, Small RNA-Seq, ribosome profiling, single cell analysis and spatial profiling), qualifying and normalizing libraries and sequencing on an Illumina instrument.
Key instrumentation within the laboratory includes an Illumina NovaSeq X, two Illumina MiSeq instruments, a 10x Genomics Chromium iX Controller, two 10x Genomics Chromium Controllers, a 10x Genomics CytAssist, a Nexcelom Cellometer K2, an Agilent 4150 TapeStation, two Covaris ultrasonnicators, and a Bio-Rad CFX Duet Real Time System. A database and LIMS system (GNomEx) has been designed to track and store experimental information and enable data distribution.
Services
- Sample Quality Assays
- Library Preparation
- Library Quality Control
- Illumina Sequencing
- Single Cell Profiling
- Spatial Profiling
Location
High-Throughput Genomics Shared Resource
Huntsman Cancer Institute Room 1753
2000 Circle of Hope
University of Utah
Salt Lake City UT 84112
Contact Us
High-Throughput Genomics Director
Brian K. Dalley, PhD
brian.dalley@hci.utah.edu
801-585-7192
High-Throughput Genomics Associate Director
Opal Allen, PhD
opal.allen@hci.utah.edu
801-581-6346
Governance
HCI Senior Director Oversight
Alana Welm, PhD
Faculty Advisory Committee Chair
Katherine Varley, PhD
Faculty Advisory Committee Members
Richard Clark, PhD
Jason Gertz, PhD
Christopher Gregg, PhD
Mei Koh, PhD
Philip Moos, PhD
Andrew Post, MD, PhD
Sean Tavtigian, PhD
Joseph Yost, PhD